Figure 4.

Absence of cell death and proliferation in the brain following irradiation and bone marrow transplantation. Tissue sections from the CNS of chimeric mice were stained at indicated time points after irradiation and BM transfer with antibodies against Ki-67 (TEC-3, DAKO, 1:20) and BrdU (in situ Detection Kit, BD PharMingen) for proliferation and TUNEL (Roche) for apoptotic cells. Spleens of chimeric mice were used as positive controls (insets). Representative pictures (cerebellum) from each experimental group (n = 2–3 mice) are shown.