Expression of CNG channel subunits in the mouse spinal cord after intraplantar zymosan injection. Data are expressed as mRNA ratio of zymosan-injected mice (tissue extracted 4, 8, and 24 h after injection) relative to untreated control mice whose mRNA expression levels were set to 1 (indicated as a dotted line). CNGA3 mRNA was significantly upregulated at 4 and 8 h after zymosan injection, whereas mRNA of CNGA2, CNGA4, CNGB1, and CNGB3 was not affected. CNGA1 mRNA was not detected. The quantity of CNG channel subunit mRNA relative to 18S mRNA was calculated by the 2−ΔΔCT method. n = 4–6 per group. All data are presented as mean ± SEM. *p < 0.05.