Figure 3.
Generation of the inducible DATCreERT2 transgenic mouse line. A, B, Brain sections of the transgenic reporter line ROSA26 expressing the inducible Cre-recombinase injected with either oil or tamoxifen. Whereas in mice injected only with oil, no activity of the reporter gene is detected (A), mice injected with tamoxifen show specific activity of the reporter in the mesencephalic region (B). C, D, The activity of the Cre recombinase is restricted to DA neurons, as determined by X-gal staining (blue) in combination with TH immunohistochemistry (brown). The inset (D) shows a higher magnification of double-stained neurons (arrows). Scale bars: A, B, 150 μm; C, 50 μm.