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. 2011 Jan 12;31(2):644–649. doi: 10.1523/JNEUROSCI.2006-10.2011

Figure 1.

Figure 1.

BDNF increases KCC2 expression in immature hippocampal cultures. A, Representative Western blot analysis and quantification of KCC2 expression in dissociated hippocampal cultures treated with BDNF (50 ng/ml) (n = 3–7). Dissociated cultures were treated with BDNF at DIV 1, DIV 8, and DIV 15, and analyzed 3 d after the treatment. Data are normalized with respect to the average in nontreated controls of corresponding age. *p < 0.05, **p < 0.01, one-sample t test. Error bars represent SEM. βtub, β-tubulin. B, Representative immunofluorescent staining and quantification of KCC2 expression in dissociated hippocampal cultures treated with BDNF (50 ng/ml) (n = 119–125). Neurons were treated with BDNF at DIV 6–8 and analyzed 24 h later. Data are normalized to the average in nontreated controls. ***p < 0.001, one-sample t test. Error bars represent SEM. Scale bar, 40 μm. C, KCC2b and KCC2a mRNA levels in DIV 6–8 cultured neurons 1–2 h after BDNF (50 ng/ml) application as detected by real-time PCR (n = 10). Values of corresponding nontreated controls were set to 100%. *p < 0.05, not significant (n/s); p > 0.05, one-sample t test. Error bars represent SEM.