Fig 6. The inhibition of ZIKV infection by TRIM56 is independent of the biogenesis of or regulation by host miRNAs.

(A) Expression of miR-92a and miR-21 in control HEK293T cells (WT) and two Dicer-knockout clonal cell lines (No-Dice (2–20) and (4–25)) was determined by qPCR and normalized to U6 snRNA. Results were representative of two independent experiments. (B and C) WT HEK293T and No-Dice cells were infected by ZIKV MR766 strain (MOI = 1) for indicated time points, followed by qPCR analysis of the kinetics of intracellular ZIKV RNA replication (B) and TCID₅₀ assay of progeny virus production in culture supernatants at 72 h.p.i. (C). Student t-test, *P<0.05. Results were reproduced three times. (D-F) WT HEK293T and No-Dice (4–25) cells were stably transduced with empty vector (Bsr) or Flag-T56 and subsequently infected by ZIKV MR766 strain (MOI = 1) for 72 h. Intracellular expression of viral E and NS5 proteins, action, and Flag-T56 were analyzed by immunoblotting (D), and intracellular ZIKV RNA levels was quantified by qPCR (E). Student t-test, **P<0.01. Progeny virus production in culture supernatants was measured by TCID₅₀ assay (F). Student t-test, **P<0.01. Results in panels D to F were reproduced three times.