Figure 4.
MOR agonist DAMGO reduced the rate of mEPSCs in rat spinal lamina I neurons also under resting Ca2+ concentrations. A, Representative traces of mEPSCs recorded before (control), during, and after (wash) DAMGO (100 nm) application, demonstrating a reduction of mEPSC rates (VHold = −70 mV). TTX (1 μm) was included in the bath solution. B, Bar graphs show the mean rates and amplitudes of mEPSCs before, during, and after application of 100 nm DAMGO. C, Effect of 10 μm DAMGO on the mean rate and amplitude of mEPSCs. Calibration: A, 50 pA, 1 s. Number of neurons tested: B, n = 4; C, n = 7. *p < 0.05; **p < 0.01.