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. 2011 Jan 26;31(4):1528–1538. doi: 10.1523/JNEUROSCI.3983-10.2011

Figure 3.

Figure 3.

Overexpression of the microtubule-binding domain of CLIPs (MBD-CLIP) leads to the formation of multiple mature axons. A, Neurons were transfected with either GFP or GFP-tagged MBD-CLIP, fixed at 2 DIV, and stained for the axonal marker Tau-1. Scale bar: 20 μm. Arrowheads indicate axons. B, Scheme of the microtubule-binding domain construct MBD-CLIP. C, Quantification of GFP-transfected (black bars) or MBD-CLIP-transfected (white bars) neurons having one (left) and multiple (right) Tau-1-positive processes (n > 247 cells from three independent cultures for each data point; *p < 0.05, ***p < 0.001). D, MBD-CLIP-transfected neurons at 11 DIV stained for GFP, Synapsin, and MAP2. Asterisk labels a GFP-positive neuron; arrowheads indicate axons. Scale bar: 50 μm. a–c, Enlarged images of regions marked in C. Scale bars: 20 μm (a) and 10 μm (b, c).