Skip to main content
. 2011 Jan 26;31(4):1528–1538. doi: 10.1523/JNEUROSCI.3983-10.2011

Figure 4.

Figure 4.

Microtubule structure in DN-CLIP-transfected or MBD-CLIP-transfected neurons. A–E, Neurons were transfected with GFP (A, B), GFP-tagged DN-CLIP (C, D), or GFP-tagged MBD-CLIP (E), fixed at 2 DIV, and stained for α-tubulin. Microtubules of DN-CLIP-transfected neurons are splayed out at the process tips, both in axons and in minor neurites of stage 2 (C) and stage 3 (D) neurons. MBD-CLIP-transfected neurons contain strongly bundled microtubules (E). Enlarged images (a1–e2) show regions marked in A–E. Scale bars (A–E), 20 μm; Enlarged images, 10 μm. F, Quantification of splayed microtubule length in axons (black bars) and minor neurites (white bars) of GFP-transfected, DN-CLIP-transfected, or MBD-CLIP-transfected neurons. Splayed microtubule length was defined as distance from microtubule tip to area of bundled microtubules (n > 160 cells from three independent cultures for each data point; **p < 0.01, ***p < 0.001).