Figure 9.
5-HT-induced [Ca2+]i increase in primary DRG neurons is inhibited by SB206553. Primary DRGs were taken from 5-HT-injected or SB-5-HT-injected mice at 30 min after injection and cultured for 12∼16 h. Cultured neurons were stimulated with 1 μm 5-HT, followed by calcium imaging. Shown are time courses of intracellular calcium signals after addition of 1 μm 5-HT in 5-HT-injected contralateral DRG neurons (A, B) and ipsilateral DRG neurons (C, D). E, Histograms showing fold change of peak value in pattern 1. In 5-HT-injected group, six independent experiments were done, and three mice were used in each experiment (total 18 mice). Totals of 88 neurons and 94 neurons were counted in the contralateral side and in the ipsilateral side, respectively. In SB-5-HT-injected group, seven independent experiments were done, and three or four mice were used in each experiment (total 26 mice). Totals of 79 neurons and 88 neurons were counted in the contralateral side and in the ipsilateral side, respectively.