Figure 2.
The PI3K inhibitor LY294002 blocks the insulin-induced increase of the DA release-enhancing effect of cocaine in NAc slices. Slices, incubated with [3H]DA (in the presence of 3 μm desmethylimipramine), were superfused and stimulated electrically at t = 40 min for 10 min. Insulin (10 nm), 1 μm cocaine (COC) and 10 μm LY294002 (LY) were added to the superfusion medium 20 min before depolarization and were present until the end of the experiment. Data are expressed as percentage of respective control (resp. control) [3H]DA release in excess of spontaneous tritium efflux, i.e., evoked release in the absence and presence of insulin. Data represent means ± SEM of 24 observations. *p < 0.01, significantly different from the effect of cocaine in the absence of insulin.