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. 2011 Oct 5;31(40):14296–14307. doi: 10.1523/JNEUROSCI.2890-11.2011

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Copyright © 2011 the authors 0270-6474/11/3114296-12$15.00/0

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Figure 7. — Inhibition of Elk-1 phosphorylation and cocaine-induced behavioral adaptations. Spontaneous locomotor activity: TDE compared with SCR injected at the dose of 8 mg/kg 60 min before the exposure to a novel environment (actimeter) does not alter either the spontaneous horizontal (ambulation) or vertical activity (rearing). B, Locomotor habituation. TDE compared with SCR injected at the dose of 8 mg/kg 60 min before the exposure to the same environment (actimeter) for 2 consecutive days does not alter the locomotor habituation. C, After 3 habituation days (days −2 to 0), mice were pretreated daily for 5 consecutive days, with either TDE or SCR at the dose of 8 mg/kg 60 min before saline (left) or cocaine (10 mg/kg; right) injection, and locomotor activity was measured. This procedure was also repeated on day 12 after a 7 d withdrawal. D, E, CPP induced by cocaine (n = 8 mice per group) was performed from mice pretreated during the conditioning phase of the protocol with TDE or its scramble version 1 h before cocaine administration in a one-pairing (D, left; E, F) or three-pairing (D, right; G, H) CPP paradigm. C, Data (means ± SEM; n = 14–15 mice per group) were analyzed using mixed-factor two-way ANOVA (repeated measure over time): saline: effect of time, F_(8,224) = 7.53, p < 0.001; effect of pretreatment, F_(1,224) = 0.35, NS; 10 mg/kg cocaine: effect of time, F_(8,216) = 10.69, p < 0.001; effect of pretreatment, F_(1,216) = 6.55, p < 0.05, followed by post hoc comparisons (Bonferroni's test). *p < 0.05, **p < 0.01, present day versus day 1; °p < 0.05, °°p < 0.01, TDE pretreated cocaine group versus SCR pretreated cocaine group. D, Data (means ± SEM; n = 8 mice per group) were analyzed using mixed-factor two-way ANOVA (repeated measure over conditioning session): one-pairing protocol SCR pretreatment: effect of conditioning, F_(1,14) = 7.37, p < 0.05; effect of treatment, F_(1,14) = 3.12, NS; one-pairing protocol TDE pretreatment: effect of conditioning, F_(1,14) = 2.51, NS; effect of treatment, F_(1,14) = 0.62, NS; three-pairing protocol SCR pretreatment: effect of conditioning, F_(1,14) = 32.68, p < 0.001; effect of treatment, F_(1,14) = 7.73, p < 0.05; three-pairing protocol TDE pretreatment: effect of conditioning, F_(1,14) = 21.0, p < 0.001; effect of treatment, F_(1,14) = 5.35, p < 0.05, followed by post hoc comparisons (Bonferroni's test). **p < 0.01, ***p < 0.001, posttest versus pretest; °p < 0.05, °°°p < 0.001, saline versus cocaine. E–H, Data (means ± SEM; n = 8 mice per group) were analyzed using paired t test for all of the groups before (Pre) and after (Post) conditioning. *p < 0.05, **p < 0.01, ***p < 0.001, drug-paired chamber versus vehicle-paired chamber. Dashed lines represent equal exploration of both chambers.