Figure 4.

Activation of AVPV GABA inputs to GnRH neuron can inhibit or stimulate GnRH neuron excitability. A, Voltage traces showing overlaid action potentials generated in a GnRH neuron following fifteen 0.33 Hz activations of the AVPV. Top shows control condition where the 15 activations generate 7 action potentials. Treatment with CNQX plus AP5 results in increased excitability with all 15 activations generating action potentials with reduced latency variation. Subsequent addition of a GABA_A receptor antagonist, gabazine (GBZ) reduces the number of action potentials to one while wash of all drugs brings the cell back to the control state (bottom). B, Voltage traces showing overlaid action potentials generated in a GnRH neuron following five 0.33 Hz activations of the AVPV. Treatment with gabazine increases the ability of GnRH neurons to follow individual AVPV stimulations.