Figure 3.
α1HA-F64C coassembles with α1 subunit in the same GABAAR. A, Single plane confocal images of HEK293 cells overexpressing α1HA-F64Cβ1γ2 receptors and EGFP probed with anti-α1- and anti-HA antibodies as indicated. Scale bar, 10 μm (n = 15). B, Heteromerization of α1 and α1HA-F64C within the same receptor. Membrane fractions of HEK293 cells transfected with α1α1HA-F64Cβ1γ2 (INPUT) were immunoprecipitated with the anti-HA antibody (IP anti-HA) and immunoblotted with the anti-α1 (top) or anti-HA antibody (bottom) (n = 3).