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Determination of the enantiopurity of synthetic S-ketamine by HPLC with UV detection (254 nm). A, Asymmetrically synthesized S-ketamine (retention time, 26.733 s) exhibited excellent selectivity (>99.9%). B, The racemate contained equal amounts of S- (retention time, 26.683 s) and R-ketamine (retention time, 23.35 s).
