Malondialdehyde and hydroxyproline levels are not significantly changed in SVCT2+/− compared to wild-type peripheral nerves. To assess the level of proline hydroxylation and lipid peroxidation, hydroxyproline and malondialdehyde assays were performed. A, Hydroxyproline assays, performed by spectrophotometry after reaction with chloramine T and Ehrlich's reagent, showed no significant differences between SVCT2+/− and wild-type sciatic nerves (P60: p = nonsignificant, n = 3; P300: p = nonsignificant, n = 3). B, Malondialdehyde concentrations, as a measure for lipid peroxidation, were not significantly different between SVCT2+/− and wild-type controls (P60: p = nonsignificant, n = 3; P300: p = nonsignificant, n = 3).