Figure 12.

Double-labeling postembedding immunogold for AMPA receptor and GABA_ARα1 in BF synapses at P12. A, B, Low- (A) and high-power (B) views of a BF terminal forming symmetrical contact with flat somatic surface and asymmetrical contact with somatic spine (asterisks). Consecutive images in B₁–B₃ demonstrate that clusters of immunogold particles for AMPA receptor (ϕ = 10 nm; arrowheads) are juxtaposed with GABA_ARα1 clusters (ϕ = 20 nm; arrows) on the PC soma under the same BF terminal. Scale bars: A, B₃ (for B₁–B₃), 500 nm. PCS, Purkinje cell soma.