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. 2011 Nov 23;31(47):17017–17027. doi: 10.1523/JNEUROSCI.2815-11.2011

Figure 2.

Figure 2.

Control of anterior–posterior epithelial identity via Odd and Slp is upstream of class IV neuron formation. A, Ventral embryonic epithelium of st11 embryos stained with fluorescent antibodies to detect Sens (green), and Slp (magenta). Note 1: Not all of SOP1a–4a form at the same time. In this panel an arrowhead indicates the approximate region where SOP3 will form. B, Future ventral epithelium of a st6 embryo after transition from a 7 to 14 stripe pattern, it is stained with fluorescent antibodies to detect Odd (green), and Slp (magenta). Each forming parasegment consists of a posterior Slp-expressing domain. Anterior and adjacent to this is an Odd-expressing domain. Anterior to the Odd-expressing domain is an unlabeled domain that represents the (Engrailed) domain where Hedgehog expression is induced (Saulier-Le Dréan et al., 1998; Nasiadka and Krause, 2002). Background staining in the green channel marks the cell membrane and so indicates the position of the cell boundaries. C, Ventral embryonic epithelium of a st12 embryo stained with fluorescent antibodies to detect Sens (green), Wg (red), and β-galactosidase to detect hh-lacZ expression (blue). D, Ventral MD neuron cluster of a WT st16 embryo, labeled to detect Elav (green), Kn (blue), and Lbe (red). E, st16 odd embryos, showing an increase in posterior-derived MD neurons. F, st16 slp embryos have duplications of anterior-derived class IV neurons, and concurrent loss of posterior-derived neurons. Note 2: In both odd and slp embryos alternate parasegments have a stronger phenotype reflecting the double-segment periodicity bias of the activity of these factors. The quantifications reported in the results section are the average across all parasegments. G, st12 odd embryos labeled to detect Sens (green) and Wg (red). There is a mirror-image duplication of the Wg expression domain and a concurrent duplication of the SOP cells in this duplicated domain. Note 3: In our figures throughout this study, at each SOP position where we saw anti-Sens staining we detected either a single cell (the SOP, e.g., position 2 in G) or its immediate daughter cells (the IIa and IIb cells, e.g., position 3 in G). H, st12 slp embryos show loss of posterior and duplication of anterior SOPs. I, Diagrams summarizing how the control of body wall identity by odd and slp prefigures MD neuron identity; color-coding as in Figure 1. Scale bars, 10 μm. Vertical bars, The parasegment boundaries.