Figure 5.

PALS1 localizes with CRB1 and MUPP1 to the subapical region adjacent to adherens junctions at the outer limiting membrane. Immunohistochemistry on 3-month-old retinas showing confocal images of staining for proteins associated with the Crumbs complex, PALS1, CRB1, and MUPP1, and for GFAP, a Müller glial cell marker. Staining for the Crumbs complex shows fragmented staining (arrows) of PALS1, CRB1, and MUPP1 in the shPals1-RxCre retinas, which indicate the dislocalization of the Crumbs complex proteins at the SAR adjacent to adherens junctions at the OLM. GFAP staining shows increased reactive gliosis in the mutant retinas. Scale bar, 50 μm.