Figure 6.

Retinal cell specification is normal, but cell patterning is aberrant in shPals1-RxCre retinas. At P10, in RxCre control retina, the staining of PKCα (green), a bipolar cell marker, and recoverin (red), a photoreceptor cell and cone bipolar cell marker, show well separated, properly layered cells. At P10, in shPals1-RxCre retinas, even though the retina layers are folded and some photoreceptor cells are dislocated into the subretinal space, most retina cells are separated and properly located in the retina. TO-PRO-3 was used for nuclear DNA staining (blue). Scale bar, 50 μm.