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. 2011 Dec 14;31(50):18381–18390. doi: 10.1523/JNEUROSCI.3386-11.2011

Figure 5.

Figure 5.

Involvement of Gi/o-proteins in CCL2-induced potentiation of Nav1.8 currents. A, Neuronal cAMP measurement following application of CCL2 in the presence or absence of PTX. CCL2-induced inhibition of adenylyl cyclase activity is blunted by PTX pretreatment (*p < 0.05, CCL2 alone vs control; #p < 0.05, PTX plus CCL2 vs CCL2 alone; one-way ANOVA followed by Dunnett's posttest, performed in triplicate). Error bars indicate SEM. B, C, I–V curves (B) and histogram (C) showing the action of CCL2 on Nav1.8 currents (***p < 0.001 compared with Control; Student's t test) and the inhibition of this effect with PTX (250 ng/ml) pretreatment (###p < 0.001 compared with CCL2 alone; Student's t test; n = 7–11). D, Preincubation with PTX also impedes the action of CCL2 on the normalized conductance (##p < 0.01; G/Gmax). Note that the experimental conditions corresponding to control and 100 nm CCL2 have been shown previously in Figure 3.