Figure 7.

Neurons are more susceptible to MG-induced protein modifications. A, Similar levels of MG were measured in astrocytes and neurons by HPLC. Results are means ± SEM of at least 13 determinations from at least four independent experiments. Data were statistically analyzed using a Student's t test (n.s., not significantly different). B, Argpyrimidine levels were assessed by Western blotting in astrocytes and neurons. Some cultures were exposed to 0.5 mm MG for 24 h before protein extraction. Arrowheads mark proteins highly modified by argpyrimidine in astrocytes. Arrows mark neuronal proteins showing increased argpyrimidine immunoreactivity following exposure to MG. C, AGE levels were assessed by Western blotting in control cultures of astrocytes (A) and neurons (N). B, C, Representative bands are shown. Similar results were obtained in three independent experiments (n = 6).