Figure 3.

In vitro oxidative stress induces Cp to convert to acidic isoforms and total CSF protein carbonylation to increase. A, WB analysis of Cp profile in the CSF from a representative CN subject resolved by 2DE under resting conditions or after treatment with increasing amounts of H₂O₂ (1, 5, 10 mm); percentages indicate the amount of total Cp signal present in regions A and B (Fig. 1); the arrows indicate low-molecular-weight products generated by protein oxidation. B, Detection of protein carbonylation by OxyBlot assay on total CSF proteins resolved by SDS-PAGE and stained for carbonyl groups, under resting conditions or after oxidation obtained by treatment with Asc with or without ferrous chloride (FeCl₃). C, WB analysis of 2DE Cp profile in the CSF under resting conditions or after oxidation treatments as in B; percentages indicate total Cp signal present in regions A and B (Fig. 1).