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. 2019 Jun 27;8:e46267. doi: 10.7554/eLife.46267

Figure 4. Silencing of peptidyl transferase activity by XBP1u nascent chain.

(A) Conformation of C4398 in XBP1u-RNC (blue). (B) C4398 conformation in the paused complex in comparison with A-site accommodated 80S (PDB ID 5GAK, softblue) and with a post state 80S without an A-site tRNA (PDB ID 5AJ0, softpink) (C) Model of an incoming A-site tRNA (yellow, PDB ID 5GAK) clashes with Leu259 of XBP1u. Accommodation of A-site tRNA is prevented by XBP1u.

Figure 4.

Figure 4—figure supplement 1. Comparison of C4398 (C2452) and U4531 (U2585) conformation in XBP1u-RNC with other post-state ribosome 80S models.

Figure 4—figure supplement 1.

(A) State of the base C4398 and U4531 in XBP1u-RNC (blue). (B) State of C4398 and U4531 compared with post state 80S (PDB ID 5AJ0, softpink and 5LZS, softblue) without an accommodated A-site tRNA. (C) - (D) (A) and (B) displayed with isolated density for the base C4398 (XBP1u-RNC in blue, EMD ID 2875, softpink and 4130, softblue).