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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Microsc Microanal. 2019 May 14;25(4):942–949. doi: 10.1017/S1431927619000606

Figure 3. Kinetic assay for hemifusion between influenza VLPs and 5% DiD liposomes.

Figure 3

Vesicles and VLPs are incubated in a 37 C plate reader, and fusion is stimulated by acidification to pH 5.1 at time = 0 s. The acidification results in hemifusion between VLPs and liposomes, dequenching the dye as it is distributed into a larger lipid pool.