Skip to main content
. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Microsc Microanal. 2019 May 14;25(4):942–949. doi: 10.1017/S1431927619000606

Figure 4. Cryo-CLEM images of different types of on-grid events and their corresponding fluorescence signals.

Figure 4

A: Targeting images are used to define points of interest (yellow boxes). Left: fluorescence-based targeting of potential hemifusion sites. Right: EM-based targeting of VLPs and vesicles irrespective of fluorescence. Scale bars = 2 μm. B: High-magnification micrographs are taken within the targeted areas. Scale bars = 50 nm. C: During post-processing, fluorescence and EM maps are correlated with greater precision, and fluorescence intensities are quantified for all imaging locations. Roman numerals correspond to the high-magnification images in B. Scale bars = 2 μm.