Fig. 1.

The crystal structure of the extended CtRea1-MIDAS reveals novel Rea1-specific elements. a Domain architecture of Rea1. The domain boundaries are indicated by the residue numbers below for S. cerevisiae (black) and C. thermophilum (red). b Crystal structure of the Rea1-MIDAS domain from C. thermophilum in two orientations. The close-up view highlights the DxSxS motif required for coordination of the divalent metal ion (left-hand structures). A ConSurf analysis of the CtRea1-MIDAS domain is shown in the two right-hand structures. Conserved amino acids, maroon; variable amino acids, turquoise. c Structure comparison of the CtRea1-MIDAS domain and the integrin A-domain (PDB ID: 1IDO) in two orientations. The integrin A-domain, green; the classical MIDAS fold of Rea1, blue; Rea1-specific elements I–III, yellow, purple, and red, respectively