Fig. 4. cPLA2 is activated and present at lysosomal membranes after SCI.

a–f The protein expression of activated (phosphorylated, p-cPLA2) and total cPLA2 in the cytosol and the lysosome-enriched fractions isolated from sham control and injured spinal cords at 2 and 24 h post injury. Each lane represents an individual animal. Quantitative analysis of Western blot for the phosphorylation rate of cPLA2 (i.e., ratio of p-cPLA2/cPLA2) are indicated in b, c, e, and f. Data are mean ± SEM, Mann–Whitney test (two-tailed), n = 6 from 12 mice/group. *p < 0.05, **p < 0.01 versus Sham. g, h Expression of p-cPLA2 and cPLA2 at purified lysosomes from sham control and injured spinal cords at 2 and 24 h. Data are mean ± SEM, One-way ANOVA, Tukey post hoc analysis. n = 6 from 12 mice/group. *p < 0.05 versus Sham. i cPLA2 enzymatic activity assay was performed in the purified lysosomes at 2 and 24 h after SCI. Data are mean ± SEM, One-way ANOVA with Tukey post hoc analysis, n = 6 mice/group; **p < 0.001