Fig. 4. HeLa cells can resist emodin treatment.
a Representative images showing the TIFs in control and 5 μM emodin-treated HeLa cells (48 h, 10 and 40 days). γ-H2AX (red), telomere (green). Nuclei were stained with DAPI (blue). White arrow heads indicate the TIFs. Scale bars are 2 μm. b The graph showing the percentage of TIFs with different treatment. c Representative images of metaphase telomere FISH. HeLa cells were treated with emodin in 5 μM for 48 h, 10 and 40 days. White arrows indicate MTS and yellow arrows indicate SFE. Scale bars are 5 μm. d, e Percentage of chromosomes with MTS and SFE for different treatment. f Representative images of TUNEL-positive percentage of apoptosis cells were detected by TUNEL with indicated condition. Scale bars are 20 μm. g Graph showing the percentage of TUNEL-positive cells after emodin treatment. Data are shown as mean ± SD. n = 3