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. 2019 Jul 11;2:258. doi: 10.1038/s42003-019-0513-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Analysis and characterization of FAMS-isolated mitochondria. a Mitochondria sorted from liver tissue exhibited intact outer and inner membranes, as well as intact cristae structures, when evaluated by scanning electron microscopy (SEM). Scalebar, 500 nm. b mtDNA was detected by DAPI-positive fluorescence in MTR-positive liver tissue lysates stained following fixation. c Size-gated, MTG-positive events (FAMS replicates A–C) expressed the mtDNA encoded genes, mt-Nd1, mt-Nd2, mt-Nd5, and mt-Nd6, but not Tert, a nuclear-encoded gene. Mitochondria isolated using a commercially available isolation kit (differential centrifugation) exhibit variable mtDNA purity. NTC, ‘no template’ control. d ATP generation by MTG-positive events after addition of ADP without and with carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) treatment. ND none detected; mean ± SEM, n = 3 (**P < 0.01). e Volcano plot of nLC-MS/MS-identified proteins from size-gated MTG-positive events collected from liver and brain tissues; colors indicate enrichment in either liver (purple) or brain (blue)