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. 2019 Jul 11;2:258. doi: 10.1038/s42003-019-0513-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — FAMS analysis of mitochondrial subpopulations in mouse liver tissue via TOM20 and TRAP1 antibody labeling. a Mouse liver cells were stained with MTG followed by labeling with anti-TOM20, anti-TRAP1, and AlexaFluor 568 or DyLight 647-conjugated secondary antibodies, respectively. Lysates were analyzed for TOM20 and TRAP1 colabeling of mitochondria using only a 0.45–2 μm size parental gate, or b through the 0.45–2 μm size gate in addition to a parental gate for MTG-positive events