Fig. 2. LINC81507-overexpression inhibits proliferation and metastasis in vivo.
a Representative images of the xenograft tumors. b The relative volumes of tumors were analyzed. c Representative images of HE and immunohistochemical staining for Ki67 expression in tumor tissues, ×20 are shown. d LINC81507-overexpression inhibits A549 cells metastasis in vivo (n = 3). Representative photographs of gross lungs (left), with arrows pointing to lung surface tumor nodules, and the bioluminescent change in A549LINC81507-OE group was significantly decreased compared with the vector control (right), the relative tumor nodules and high metabolic area were showed. The quantitation of lung metastasis was assessed by bioluminescence measurements. e H&E-stained section of lung, with original magnification: ×4, ×10 are shown. Micrograph indicates the magnified morphology of tumor tissues. Representative images of immunohistochemical staining for CAV1, E-cadherin, N-cadherin, β-catenin, and Vimentin expression in mouse metastasis lung tissues compared with normal mouse lung tissues (in ×10 and ×40). Data were represented as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001