Fig. 3. LINC81507 overexpression inhibits lung cancer cells’ proliferation and its location in tissues and cells.

a Assessment of proliferation of A549, PC-9, and Calu-1 cells transfected with pcDNA3.1-LINC81507 or negative control by CCK-8 assay. b 5-ethylnyl-2ʹ-deoxyuridine (EdU) incorporation assay of A549, PC-9, and Calu-1 cells transfected with pcDNA3.1-LINC81507 or negative control. c Colony formation assay of A549, PC-9, and Calu-1 cells transfected with pcDNA3.1-LINC81507 or negative control. d Fluorescence in situ hybridization (FISH) assay of LINC81507 location in NSCLC tissues. After nuclear and cytosolic separation, RNA expression levels were measured by RT-qPCR. GAPDH was used as a cytosol marker and U6 was used as a nucleus marker. Data were represented as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. LINC81507 OE means LINC NONHSAT081507.1 overexpression. WT means wild-type cell which receive no treat. NC ctrl stands for normal cell control