Fig. 5. The relation between LINC81507 and miR-199b-5p.

a Relative expression of the three indicated miRNAs from more than 20 NSCLC tumor tissues and the matched adjacent normal tissues. b The expressions of miR-199b-5p were analyzed using RT-qPCR in NSCLC cells. c The expressions of miR-199b-5p were analyzed using RT-qPCR in NSCLC cells transfected with pcDNA3.1-LINC81507 or negative control. d The expressions of CAV1 in NSCLC cells after decreasing miR-199b-5p. e The expressions of indicated mRNAs from more than 20 NSCLC. f Migration assay of A549 and Calu-1 cells co-transfected with pcDNA3.1-LINC81507, negative control, miRNA-199b inhibitor or miRNA-199b-5p mimic. g Related expression of CAV1 protein in A549 and Calu-1 cells after co-transfected with pcDNA3.1-LINC81507, negative control, miRNA-199b inhibitor or miRNA-199b-5p mimic was determined by western blot analysis. h Schematic of LINC81507 wild-type (WT)/mutant (Mut) and pGL3-CAV1 3′-UTR Mut/WT luciferase reporter vectors are illustrated, and the relative luciferase activities were analyzed in A549 cells co-transfected with miR-199b-5p mimics or miR-NC and luciferase reporter vectors pcDNA3.1-LINC81507-WT or Mut. i The RIP assay revealed that LINC81507 and miR-199b-5p were enriched in the same Ago2-immunoprecipitate. Data were represented as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001