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. Author manuscript; available in PMC: 2019 Jul 12.
Published in final edited form as: Arch Toxicol. 2014 Mar 13;88(9):1749–1763. doi: 10.1007/s00204-014-1223-9

Figure 5: miR-34a regulates the expression of Met and Gas1 in a dose-dependant manner.

Figure 5:

(A) miR-34a, Met and Gasl expression in C3H10T1/2 cells 24 hours following miR-34a low (L; 4.9 fold) and high (H; 18.3 fold) transfections. Fold change was calculated relative to a scrambled RNA transfection and U6-snRNA or Gapdh as endogenous controls. Values are presented as mean ± SEM (N>3). (B) A region from the mouse Gas1 3’-UTRs spanning the miR-34 binding site was cloned into the psiCHECK-2 plasmid downstream of a Renilla luciferase reporter gene. This plasmid or its mutated version was transfected 24 hours after miR-34a transfection into HEK293T cells. Renilla luciferase activity was measured 24 hours later and normalized to firefly luciferase activity. Similarly, transfections with a scrambled miRNA and a mutated version (mut) of miR-34a binding site we used as controls. Values are presented as mean ± SEM (N>3). (C) An illustration of miR-34a and its binding site on the mouse Gas1 3’-UTR (or its mutated version). P-value < 0.05, 0.002 is indicated by * / **, respectively (two-sided t-test).