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. Author manuscript; available in PMC: 2019 Jul 12.
Published in final edited form as: Cell Metab. 2017 Apr 4;25(4):856–867.e5. doi: 10.1016/j.cmet.2017.03.007

Figure 7. FXR Physically Interacts with CASPASE 1 and NLRP3.

Figure 7.

(A) Co-immunoprecipitation analysis of FXR interaction with NLRP3 inflammasome in THP-1 cells.

(B) Influence of the FXR plasmid transfection.

(C) Influence of FXR silencing.

Proteins were immunoprecipitated with FXR-specific antibody or an IgG control. Precipitates were probed for FXR, NLRP3, ASC, and CASPASE 1; aliquots were blotted in parallel as a loading control. GAPDH was used as loading control in western blot analyses (n = 3).

(D) Schematic illustration of the role and mechanism of FXR/bile acids in the regulation of NLRP3 inflammasome in cholestasis-associated sepsis.