(A) Co-immunoprecipitation analysis of FXR interaction with NLRP3 inflammasome in THP-1 cells.
(B) Influence of the FXR plasmid transfection.
(C) Influence of FXR silencing.
Proteins were immunoprecipitated with FXR-specific antibody or an IgG control. Precipitates were probed for FXR, NLRP3, ASC, and CASPASE 1; aliquots were blotted in parallel as a loading control. GAPDH was used as loading control in western blot analyses (n = 3).
(D) Schematic illustration of the role and mechanism of FXR/bile acids in the regulation of NLRP3 inflammasome in cholestasis-associated sepsis.