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. 2019 Jul 2;26:101265. doi: 10.1016/j.redox.2019.101265

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© 2019 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 4 — FIH:FIH-OTUB1 heterotrimer formation. Analysis of the composition of the covalently linked FIH-OTUB1 complex by (A) blue native-PAGE (first dimension, 1D) following ectopic expression in HEK293 cells and by (B) SDS-PAGE as second dimension (2D). *, FIH-OTUB1 heterodimer; x, FIH homodimer; y, FIH monomer; arrows 1–3, monomeric FIH originating from FIH monomers [1], FIH:FIH homodimers [2] or the FIH:FIH-OTUB1 heterotrimer [3]. Data are representative for three independent experiments throughout.