FIGURE 6.
LEfSe analysis displaying predicted functional profiles of the different tissue types in P. margaritifera. The barplot shows the 21 differentially abundant KEGG pathways (level 2) identified in the microbiomes of particular host tissues, based on a Linear Discriminant Analysis (LDA, p < 0.05 for factorial Kruskal–Wallis and pairwise Wilcoxon tests). Each colour denotes the tissue type, wherein the distinguishing functional traits were identified (Gill = red, Gonad = pink, Byssus gland = gold, Mantle = green, Muscle = purple, Mucus = blue, Gut = cyan). Only KEGG pathways meeting an LDA significant threshold > 2.5 are shown. The threshold of the logarithmic LDA score is represented by the dotted line. See Supplementary Data S6 for abundances of predicted gene counts of KEGG pathways (level 1 to 3).