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. Author manuscript; available in PMC: 2020 Jul 8.
Published in final edited form as: Curr Biol. 2019 Jun 27;29(13):2157–2166.e6. doi: 10.1016/j.cub.2019.05.074

Figure 2. Evolution of a cis regulatory element of Abd-B contributes to the D. santomea phenotype.

Figure 2.

(A) Map of the genomic region between abd-A and Abd-B, with the iab-5 region highlighted in gray. The 1 kb IAB5 initiator element (red) was replaced by an eye-directed RFP cassette (orange triangle). (B) Outline of the reciprocal hemizygosity test of IAB5, showing phenotypes of representative hemizygotes. (C) Quantification of A5 segment pigmented area in male progeny of the crosses shown in (B) (Student’s t-test, ****: p<0.0001). (D, E) in situ hybridization to detect GFP expression of D. yakuba and D. santomea iab-5 GFP reporter transgenic flies at late pupal stages (93–94 hAPF). See also Figure S2.