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. Author manuscript; available in PMC: 2020 Jul 8.
Published in final edited form as: Curr Biol. 2019 Jun 27;29(13):2157–2166.e6. doi: 10.1016/j.cub.2019.05.074

Figure 6. A transposon insertion at ebony confers increased expression in the D. santomea abdomen.

Figure 6.

(A) Map of the ebony locus indicating the position of a transcription-activating abdominal enhancer (“act”) and two repressive elements, “male rep” and the intronic “stripe rep” element. Schematic of ebony full regulatory GFP reporter construct (eFR-GFP), which contains the entire upstream and intronic regions of ebony. Red triangle indicates the position of a ~500 bp helitron transposon present in D. santomea. (B) Phylogeny showing a single origin of the D. santomea ebony helitron. Genomic helitrons represent top matches in the D. yakuba, D. santomea, and D. teissieri genomes. (C-E) GFP expression patterns of transgenic reporters in the posterior body segments A5 and A6 measured 6 hours post-eclosion. (C) D. yakuba eFR-GFP. (D) D. santomea eFR-GFP. (E) D. yakuba eFR+TE-GFP construct, in which the helitron element from D. santomea was precisely cloned into the D. yakuba 5’ regulatory region. Middle and bottom images show magnified regions outlined in the top images by red and blue dashed lines. See also Figure S7 and Data S2.