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. 2019 Jul 5;10:863. doi: 10.3389/fphys.2019.00863

FIGURE 2.

FIGURE 2

Expression analysis and channel formation of human WT and ΔPEST KIR2.1 protein. (A) Western blot depicting WT (approximately 50 kDa) and ΔPEST (approximately 47 kDa) KIR2.1 proteins expressed in HEK293T cells. Non-transfected cells (NT) were used as negative control. Ponceau staining depicts loading control. (B) Subcellular localization of ectopically expressed WT and ΔPEST KIR2.1 channel proteins in COS-7 cells. Arrows indicate membrane ruffles with KIR2.1 expression. (C) HEK293T cells were co-transfected with GFP-tagged murine KIR2.1 and either WT or ΔPEST KIR2.1. Non-transfected cells (NT) were used as negative control. KIR2.1-GFP was detected by GFP antibody (WB: GFP) for IP control, and N-terminal KIR2.1 antibody (WB: KIR2.1) was used to detect KIR2.1-GFP either WT or ΔPEST non-tagged KIR2.1 protein. Positions of KIR2.1-GFP, WT-KIR2.1 and ΔPEST-KIR2.1 are indicated. Lysate blots serve as immune-precipitation input control. *IgG heavy chain.