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TSL suppresses the VEGFR2 signaling pathway. (A) HUVECs were pretreated with indicated concentrations of TSL for 30 min, followed by the addition of VEGF (25 ng/ml) for another 5 min. Phosphorylation status of VEGFR2, JNK, ERK1/2, p38 was determined by western blots. (B) Quantification of the Western blots. Data are represented as mean ± SEM (n = 3). ### P < 0.001 versus the vehicle control. *P < 0.05, **P < 0.01, ***P < 0.001 versus the vehicle + VEGF group.
