Figure 6. Comparison of different MTLa/a cell states in infection.

(A) Competitive fitness of C. albicans MTLa/a cells in a disseminated infection model. A 50:50 mix of white EFG1^+/− and gray efg1^−/− cells were co-inoculated in the GI (CAY6970/7869). Cells analyzed from fecal pellets. n=3 mice. ***, P<0.001 by t test.

(B) A 50:50 mix of white EFG1^+/− and opaque-like efg1^−/− cells were co-inoculated in the GI (CAY6970/7868) and cells analyzed from fecal pellets. n=4 mice. ***, P<0.001 by t test.

(C) A 50:50 mix of gray efg1^−/− and opaque-like efg1^−/− cells were co-inoculated in the GI (CAY7022/7868) and cells analyzed from fecal pellets. n=3 mice. ***, P<0.001 by t test.

(D) Kaplan-Meier survival curves comparing white, gray and opaque-like cells (CAY6970/7022/7023). *, P <0.05 and **, P <0.005 by log-rank test. n=9 mice each.

(E) Phenotypes of cells recovered from the kidney at 15 dpi (or when moribund) for EFG1^+/− and efg1^−/− strains and 3 dpi for EFG1^+/+ isolate. n=7 mice for EFG1^+/− and efg1^−/− strains and n=4 mice for the EFG1^+/+ isolate. Error bars, SD. ***, P<0.001 by t test compared to the inoculum.

(F) Competitive fitness of C. albicans cells in a disseminated model. A 50:50 mix of white EFG1^+/− and gray efg1^−/− cells (CAY6970/7869) were co-inoculated in the tail vein and recovered 7 dpi from the kidney. n=4 mice. ***, P<0.001 by t test.

(G) A 50:50 mix of gray efg1^−/− and opaque-like efg1^−/− cells (CAY7022/7868) were co-inoculated in the tail vein and recovered 7 dpi from the kidney. n=3 mice. ***, P<0.001 by t test.

(H) A 50:50 mix of white EFG1^+/− and opaque-like efg1^−/− cells (CAY6970/7868) were co-inoculated into the tail vein and recovered 7 dpi from the kidney. n=5 mice. ***, P<0.001 by t test.