KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-MYC antibody | Millipore | CAT# 05-724 |
| Donkey anti-mouse HRP antibody | Jackson ImmunoResearch | CAT# 715-036-151 |
| Supersignal West Pico chemiluminescent substrate | Thermo Fisher | CAT# PI34580 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Nourseothricin | Werner BioAgents | CAT# 5.010.000 |
| Chloramphenicol | Fisher | CAT# BP904 |
| Streptomycin sulfate | MP Biomedicals | CAT# 0219454180 |
| Penicillin-G potassium | Fisher Bioreagents | CAT# BP914100 |
| Sucrose | Sigma-Aldrich | CAT# S0389 |
| Dextrose | Fisher Scientific | CAT# D16-3 |
| 2.5% glutaraldehyde in 0.1 M Na-Cacodylate buffer, pH 7.4 | Electron Microscopy Sciences | CAT# 15960 |
| 0.1 M Na-Cacodylate buffer, pH 7.4 | Electron Microscopy Sciences | CAT# 11652 |
| Osmium tetroxide | Electron Microscopy Sciences | CAT# 19150 |
| Ethanol | Pharmco-Aaper | CAT# 111000200 |
| Ampicillin sodium salt | Fisher Bioreagents | CAT# BP1760 |
| Kanamycin sulfate | Fisher Bioreagents | CAT# BP906-5 |
| Doxycycline hyclate | Sigma-Aldrich | CAT# D9891 |
| Critical Commercial Assays | ||
| MasterPure Yeast RNA Purification Kit | Epicentre | CAT# MPY03100 |
| DNA Clean & Concentrator kit | Zymo Research | CAT# 11-305C |
| CHROMagar Candida medium | Fisher Scientific | CAT# NC9514149 |
| Turbo DNase | Fisher Scientific | CAT# am2238 |
| SuperScript IV Reverse Transcriptase | Thermo Fisher | CAT# 18090050 |
| iTaq Universal SYBR Green Supermix | Bio-Rad | CAT# 172-5121 |
| 4-15% TGX Strain-Free gel | Bio-Rad | CAT# 4568084 |
| Deposited Data | ||
| Raw data files for RNA sequencing | NCBI Gene Expression Omnibus | GSE105399 |
| Experimental Models: Organisms/Strains | ||
| Mouse: BALB/c | Charles River Laboratories | CAT# 028 |
| Candida albicans: full strain list is presented in Table S3 | This paper | N/A |
| Oligonucleotides | ||
| Full list of primers is presented in Table S2 | This paper | N/A |
| Recombinant DNA | ||
| Plasmid: pRB360 | This paper | N/A |
| Plasmid: PRB823 | This paper | N/A |
| Plasmid: pRB721 | This paper | N/A |
| Plasmid: pADH76 | Aaron Hernday, UCMerced | N/A |
| Plasmid: pMBL162 | (Lohse and Johnson, 2010) | N/A |
| Plasmid: pMBL165 | (Lohse and Johnson, 2010) | N/A |
| Plasmid: pSFS-13 × MYC-SAT1 | (Hernday et al., 2010) | N/A |
| Plasmid: pDis3 | (Gerami-Nejad et al., 2013) | N/A |
| Software and Algorithms | ||
| EdgeR (version 3.5) | (Robinson et al., 2010) | https://www.bioconductor.org/packages/release/bioc/html/edgeR.html |
| HTseq (version 0.9.1) | (Anders et al., 2015) | https://htseq.readthedocs.io/en/release_0.10.0/ |
| STAR (version 2.5) | (Dobin et al., 2013) | https://github.com/alexdobin/STAR |
| GO term finder | (Inglis et al., 2012) | http://www.candidagenome.org/cgi-bin/GO/goTermFinder |
| Genome Analysis Toolkit | Broad Institute | https://software.broadinstitute.org/gatk/ |
| Integrative Genomics Viewer | Broad Institute | http://software.broadinstitute.org/software/igv/ |
| BLAST | Candida Genome Database | http://www.candidagenome.org/cgi-bin/compute/blast_clade.pl |
| FlowJo (version 10.2) | FlowJo, LLC | https://www.flowjo.com/solutions/flowjo |