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. 2019 Jul 12;19:45. doi: 10.1186/s12896-019-0526-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — The purification of alkaline pectinase from B. tequilensis CAS-MEI-2-33. a Ammonium sulfate fractionation curve; b Elution curve of Mini Macro-Prep High-Q ion exchange chromatography; c. Elution curve of Sephacryl S-100 column chromatography; d. The partial purification of alkaline pectinase from B. tequilensis CAS-MEI-2-33 using TS. M: molecular weight makers; 1. Sephacryl S-100 column chromatography with pH 7.2; 2: Mini Macro-Prep High-Q ion exchange chromatography with pH 8.0; 3. Concentrated ammonium sulfate salting out solution