Fig. 1.

The GEMINI framework for identification of genetic interactions from pairwise CRISPR knockout screens. a Combinatorial CRISPR libraries are designed using unique guide pairs to target two genes. The library is then screened in a pooled format, and next-generation sequencing is performed to obtain guide pair representations at early and late time points. b Observed log-fold changes (LFCs) of guide pairs are used to infer effects across all samples (sample-independent) and effects within each sample (sample-dependent). Sample-dependent effects are then used to score and identify significant genetic interactions