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. 2019 Jul 11;47:42. doi: 10.1186/s41182-019-0171-8

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The dose-response curves of ivermectin against equine piroplasm parasites in vitro. The curve shows the correlation between relative fluorescence units (RFUs) and the log concentrations of IVM (μM) in B. caballi and T. equi treated with various concentrations of IVM. The result was determined by fluorescence assay after 96 h of incubation. The values obtained from three separate trials were used to determine the IC₅₀s using nonlinear regression (curve fitting analysis) in GraphPadPrism software