Fig. 3.

High glucose promotes proliferation and suppresses apoptosis in PC cells. a, b PC cells were treated with specific glucose levels and mannitol. At the indicated time points (24 h, 48 h and 72 h), cell viability was detected by MTT assays. c, d Effects of normal glucose, high glucose and mannitol on apoptosis in BxPc-3 and MiaPaCa-2 cells were detected by flow cytometry. e-h PC cells were treated with normal glucose, high glucose and mannitol for 48 h, and western blotting assays were performed to detect the expression of cyclin D, PCNA, Bax and Bcl-2; β-actin was used as an internal loading control. n = three independent experiments, *P < 0.05, **P < 0.01, or ***P < 0.001