Fig. 4.

High glucose increases SREBP1 expression and decreases autophagy. a-d The expression of SREBP1 was detected by western blotting assays after PC cells were cultured with normal glucose, high glucose and mannitol. e-h PC cells were treated with normal glucose, high glucose and mannitol for 6 h, and western blotting assays were performed to detect the expression of LC3, p62 and Beclin1; β-actin was used as an internal loading control. n = three independent experiments, **P < 0.01, or ***P < 0.001