Fig. 6.

High glucose regulates autophagy by upregulating SREBP1 expression. a-d After knocking down SREBP1 and culture with normal glucose and high glucose medium, the expression level of autophagy markers was detected by western blotting assays; (e) LC3 expression was detected by immunofluorescence, green represents LC3, and blue represents nuclei, scale bars = 20 μm; (f) Autophagic flux was detected via diploid adenovirus (mRFP-GFP-LC3); red dots represent autolysosomes and yellow dots autophagosomes, scale bars = 20 μm; and (e) Autophagosomes were detected by electron microscopy (black arrow shows autophagosome), scale bars = 500 nm. n = three independent experiments, ns, not significant, *P < 0.05, **P < 0.01, or ***P < 0.001