Skip to main content
. Author manuscript; available in PMC: 2020 Jun 27.
Published in final edited form as: Cell. 2019 May 30;178(1):176–189.e15. doi: 10.1016/j.cell.2019.05.003

Figure 4. LDHA-associated lactate negatively regulates RLR signaling.

Figure 4.

A–C, Measurement of lactate secretion (A), IFN-β mRNA expression (B) or protein levels (C) in Hep3B cells with control or LDHA knockdown and transfected with Poly (I:C) for 4 hours. D, Q-PCR analysis of IFN-β mRNA expression in Hep3B cells infected with control or LDHA shRNA along with or without Flag-LDHA expression and then transfected with Poly (I:C). E, Measurement of lactate secretion in HEK293 cells pretreated with or without Sodium Oxamate (20 mM) overnight. F-H, Q-PCR analysis of IFN- μ or Sev mRNA expression in HEK293 cells treated with or without sodium oxamate (20 mM) overnight and then transfected with Poly(I:C) for 2 hours or infected with Sev as indicated. I and J, Q-PCR analysis of IFN-β mRNA expression in Hep3B cells pretreated with or without sodium oxamate (20 mM) or 2-DG (2mM) and then added with or without Lactate (10 mM) before transfecting with Poly(I:C). K, Q-PCR analysis of IFN-β mRNA expression in Hep3B cells infected with control or HK2 shRNA and then added with or without Lactate (10 mM) before transfecting with Poly(I:C). L–M, Q-PCR analysis of IFN-β mRNA expression in iBMM cells cultured in mediums containing glucose (25 mM) or galactose (25 mM) and then added with or without Lactate before transfecting with Poly(I:C). N, Q-PCR analysis of IFN-β mRNA expression in Hep3B cells with control or MCT1 knockdown and then treated with or without sodium oxamate (20mM) overnight before lactate addition (10 mM) and poly(I:C) transfection. Data are means±SD. **p < 0.01. See also Figure S4 and S5.